Services

  • Training on human embryonic stem cells (hESCs)/induced pluripotent stem cells (iPSCs) culture methods (harvesting, passaging, thawing and freezing)

  • Training for reprogramming (differentiation, characterization)

  • Perform as a service (cell culture, reprogramming, differentiation, characterization, CRISPR genome editing)

  • Primary cell isolation extraction and expansion

  • Teratoma injection and extraction

  • Protocol development

If you would like to request a service that is not outlined on this page, please email hpscf@ohri.ca, and we will be happy to provide a customized quote for you. For any questions or feedback, please contact us at hpscf@ohri.ca.

  • Differentiation

We have successfully differentiated the following cell types (see below) from iPSCs and/or ESCs. Training on how to differentiate iPSCs and ESCs is available at the facility. Prices vary depending on the protocol used for differentiation.

  • Smooth Muscle Cells

  • Vascular Smooth Muscle Cells

  • Chondroprogenitors Cells

  • Cardiomyocytes

  • Neuron Progenitor Cells (NPCs)

  • Neurons

  • Neural Crest Cells

  • CRISPR/Cas9 genome editing

We have developed the protocols necessary to perform edits in pluripotent stem cells using a number of different strategies. For instance, in addition to relying on random insertions/deletions through non-homologous end joining (NHEJ) to knockout a gene of interest, we can pair a Cas9/sgRNA vector with a single-stranded DNA template for precision knockout. We have also successfully inserted large transgenes containing selection markers to knock out your gene of interest. We also have all of the tools and vectors needed for gene knock-in at the AAVS1 safe harbor locus for sustained expression. Please contact for more information.