Equipment

Core users are charged an hourly fee for access to all consumables and instruments required to culture and maintain healthy hPSC. The hourly fee ($15/ hr.) is charged on users booking of our BSC and includes the use of the following additional equipment:

  • Biological safety cabinets (4)

  • Tri-gas incubators are set to Hypoxic (3), normoxic (2), and sensitive cell incubators (1). These incubators monitor and maintain O2, CO2, humidity, and temperature that allows for consistent, reliable culturing conditions that most closely resemble in-vivo microenvironments.

  • Dissecting microscopes under Laminar flow hoods for convenient cell cleaning and manipulation. Users can manually select pluripotent colonies while maintaining sterility.

  • Controlled rate freezer to maximize cell recovery, saving time and money downstream.

  • Automated cell counter (Countess 3) with reusable slides, significantly reducing waste.

  • 24/7 access, and personal storage space at room temperature, 4⁰C and -20⁰C.

  • Additional equipment (extra charge)

Lonza Nucleofector 4D Core & X-unit

“The NucleofectorTM Technology uses a specific combination of optimized electrical parameters and cell type-specific solutions which enables transfer of a molecule directly into the cell’s nucleus. Due to this independency from cell proliferation, it allows for efficient transfection of even non-dividing primary cells (like resting T cells or neurons) and speeds up expression.” Click here for more information

  • University of Ottawa and affiliates: $40.00 per session.

  • External users: $60.00 per session.

  • External industry users: $200.00 per session

Covaris

"Versatile shearing device for DNA, RNA, and chromatin shearing, tissue homogenization, cell lysis, compound dissolution, and particle micronization." Click here for more information

  • University of Ottawa and affiliates: $25.00 per hour

  • External users: $40.00 per hour

  • External industry users: $100.00 per hour.

  • 150ul tubes = $15.00

Neon Transfection System

“The Neon Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells.” Click here for more information

  • University of Ottawa and affiliates: $40.00 per 2 hour session

  • External users: $60.00 per 2 hour session

  • External industry users: $100.00 per 2 hour session

  • Neon tips = $38.85

  • Neon Cuvette = $56.01

  • Technician time = $50.00 / hr.

  • Other equipment (please contact for pricing)

NanoPRO 1000

“A simple Western system for charge-based assays to characterize proteins.” Click here for more information

ThermoFisher Arrayscan VTI (Cellomics)

“The Thermo Scientific™ ArrayScan™ XTI Live High Content Platform is a complete research solution for automated cellular imaging analysis.” Click here for more information

Roche Light Cycler 480

“The LightCycler® 480 System is a high-performance, medium- to high-throughput PCR platform (96- or 384-well plates) that provides various methods for gene detection, gene expression analysis, genetic variation analysis, and array data validation.” Click here for more information